| Plant tissue culture is developed on the basis of plant physiology, and is the basis and important part of modern biotechnology. This paper reviews the development process of plant tissue culture in three stages: exploration, foundation and rapid development. It summarizes the application and existing problems of plant tissue culture in seedling rapid propagation, virus-free seedling cultivation,breeding, secondary metabolite development and germplasm resources preservation. With the continuous exploration of plant tissue culture theory and practice, open tissue culture technology, sugar-free tissue culture technology and new light source are becoming the development trend of new tissue culture technology. This paper introduces the advantages and research progress of these three new technologies,aiming to provide help for technicians engaged in plant tissuec ulture. |